Configure

Apollo Configuration

Apollo includes some basic configuration parameters that are specified in configuration files. The most important parameters are the database parameters in order to get Apollo up and running. Other options besides the database parameters can be configured via the config files, but note that many parameters can also be configured via the web interface.

Note: Configuration options may change over time, as more configuration items are integrated into the web interface.

Main configuration

The main configuration settings for Apollo are stored in grails-app/conf/Config.groovy, but you can override settings in your apollo-config.groovy file (i.e. the same file that contains your database parameters). Here are the defaults that are defined in the Config.groovy file:

// default apollo settings
apollo {
  default_minimum_intron_size = 1
  history_size = 0
  overlapper_class = "org.bbop.apollo.sequence.OrfOverlapper"
  track_name_comparator = "/config/track_name_comparator.js"
  use_cds_for_new_transcripts = true
  user_pure_memory_store = true
  translation_table = "/config/translation_tables/ncbi_1_translation_table.txt"
  is_partial_translation_allowed = false // unused so far
  get_translation_code = 1
  sequence_search_tools = [
    blat_nuc: [
      search_exe: "/usr/local/bin/blat",
      search_class: "org.bbop.apollo.sequence.search.blat.BlatCommandLineNucleotideToNucleotide",
      name: "Blat nucleotide",
      params: ""
    ],
    blat_prot: [
      search_exe: "/usr/local/bin/blat",
      search_class: "org.bbop.apollo.sequence.search.blat.BlatCommandLineProteinToNucleotide",
      name: "Blat protein",
      params: ""
      tmp_dir: "/opt/apollo/tmp" //optional param, uses system tmp dir by default
    ]
  ]    



  splice_donor_sites = [ "GT" ]
  splice_acceptor_sites = [ "AG"]
  gff3.source= "." bootstrap = false

  info_editor = {
    feature_types = "default"
    attributes = true
    dbxrefs = true
    pubmed_ids = true
    go_ids = true
    comments = true
  }
}

These settings are essentially the same familiar parameters from a config.xml file from previous Apollo versions. The defaults are generally sufficient, but as noted above, you can override any particular parameter in your apollo-config.groovy file, e.g. you can add override configuration any given parameter as follows:

grails {
  apollo.get_translation_code = 1
  apollo {
    use_cds_for_new_transcripts = true
    default_minimum_intron_size = 1
    get_translation_code = 1  // identical to the dot notation
  }
}

Logging configuration

To over-ride the default logging, you can look at the logging configurations from Config.groovy and override or modify them in apollo-config.groovy.

log4j.main = {
    error 'org.codehaus.groovy.grails.web.servlet',  // controllers
          'org.codehaus.groovy.grails.web.pages',    // GSP
          'org.codehaus.groovy.grails.web.sitemesh', // layouts
           ...
    warn 'grails.app'
}

Additional links for log4j:

Advanced log4j configuration: http://blog.andresteingress.com/2012/03/22/grails-adding-more-than-one-log4j-configurations/
Grails log4j guide: http://grails.github.io/grails-doc/2.4.x/guide/single.html#logging

Canned comments

Canned comments are configured via the admin panel on the web interface, so they are not currently configured via the config files.

View your instances page for more details e.g. http://localhost:8080/apollo/cannedComment/

Search tools

Apollo can be configured to work with various sequence search tools. UCSC's BLAT tool is configured by default and you can customize it as follows:

sequence_search_tools = [
  blat_nuc: [
    search_exe: "/usr/local/bin/blat",
    search_class: "org.bbop.apollo.sequence.search.blat.BlatCommandLineNucleotideToNucleotide",
    name: "Blat nucleotide",
    params: ""
  ],
  blat_prot: [
    search_exe: "/usr/local/bin/blat",
    search_class: "org.bbop.apollo.sequence.search.blat.BlatCommandLineProteinToNucleotide",
    name: "Blat protein",
    params: "",
    tmp_dir: "/opt/apollo/tmp" //optional, uses system tmp dir by default
  ]
  your_custom_search_tool: [
    search_exe: "/usr/local/customtool",
    search_class: "org.your.custom.Class",
    name: "Custom search"
  ]
]

When you setup your organism in the web interface, you can then enter the location of the sequence search database for BLAT.

Note: If the BLAT binaries reside elsewhere on your system, edit the search_exe location in the config to point to your BLAT executable.

Data adapters

Data adapters for Apollo provide the methods for exporting annotation data from the application. By default, GFF3 and FASTA adapters are supplied. They are configured to query your IOService URL e.g. http://localhost:8080/apollo/IOService with the customizable query

data_adapters = [[
  permission: 1,
  key: "GFF3",
  data_adapters: [[
    permission: 1,
    key: "Only GFF3",
    options: "output=file&format=gzip&type=GFF3&exportGff3Fasta=false"
  ],
  [
    permission: 1,
    key: "GFF3 with FASTA",
    options: "output=file&format=gzip&type=GFF3&exportGff3Fasta=true"
  ]]
],
[
  permission: 1,
  key : "FASTA",
  data_adapters :[[
    permission : 1,
    key : "peptide",
    options : "output=file&format=gzip&type=FASTA&seqType=peptide"
  ],
  [
    permission : 1,
    key : "cDNA",
    options : "output=file&format=gzip&type=FASTA&seqType=cdna"
  ],
  [
    permission : 1,
    key : "CDS",
    options : "output=file&format=gzip&type=FASTA&seqType=cds"
  ]]
]]

Default data adapter options

The options available for the data adapters are configured as follows

type: GFF3 or FASTA
output: can be file or text. file exports to a file and provides a UUID link for downloads, text just outputs to stream.
format: can by gzip or plain. gzip offers gzip compression of the exports, which is the default.
exportSequence: true or false, which is used to include FASTA sequence at the bottom of a GFF3 export

Supported annotation types

Many configurations will require you to define which annotation types the configuration will apply to. Apollo supports the following "higher level" types (from the Sequence Ontology):

sequence:gene
sequence:pseudogene
sequence:transcript
sequence:mRNA
sequence:tRNA
sequence:snRNA
sequence:snoRNA
sequence:ncRNA
sequence:rRNA
sequence:miRNA
sequence:repeat_region
sequence:transposable_element

Apache / Nginx configuration

Oftentimes, admins will put use Apache or Nginx as a reverse proxy so that the requests to a main server can be forwarded to the tomcat server. This setup is not necessary, but it is a very standard configuration.

Note that we use the SockJS library, which will downgrade to long-polling if websockets are not available, but since websockets are preferable, it helps to take some extra steps to ensure that the websocket calls are proxied or forwarded in some way too. If you are using tomcat 7, please make sure to use the most recent stable version, which supports web sockets by default. Using older versions (e.g. 7.0.26) websockets may not be included by default and you will need to include an additional .jar file.

Apache Proxy

The most simple setup on apache is as follows.. Here is the most basic configuration for a reverse proxy:

ProxyPass  /apollo http://localhost:8080/apollo
ProxyPassReverse  /apollo http://localhost:8080/apollo

Note: that a reverse proxy does not use ProxyRequests On (which turns on forward proxying, which is dangerous)

Also note: This setup will use downgrade to use AJAX long-polling without the websocket proxy being configured.

To setup the proxy for websockets, you can use mod_proxy_wstunnel, first load the module

LoadModule proxy_wstunnel_module libexec/apache2/mod_proxy_wstunnel.so

Then add extra ProxyPass calls for the websocket "endpoint" called /apollo/stomp

ProxyPass /apollo/stomp  ws://localhost:8080/apollo/stomp
ProxyPassReverse /apollo/stomp ws://localhost:8080/apollo/stomp

Debugging proxy issues

Note: if your webapp is accessible but it doesn't seem like you can login, you may need to customize the ProxyPassReverseCookiePath

For example, if you proxied to a different path, you might have something like this

ProxyPass  /testing http://localhost:8080
ProxyPassReverse  /testing http://localhost:8080
ProxyPassReverseCookiePath / /testing

Then your application might be accessible from http://localhost/testing/apollo

Nginx Proxy (from version 1.4 on)

Your setup may vary, but setting the upgrade headers can be used for the websocket configuration http://nginx.org/en/docs/http/websocket.html

    map $http_upgrade $connection_upgrade {
        default upgrade;
        ''      close;
    }


    server {
        # Main
        listen   80; server_name  myserver;

        # http://nginx.org/en/docs/http/websocket.html
        location /ApolloSever {
            proxy_http_version 1.1;
            proxy_set_header Upgrade $http_upgrade;
            proxy_set_header Connection $connection_upgrade;
            proxy_pass      http://127.0.0.1:8080;
        }
    }

Upgrading existing instances

There are several scripts for migrating from older instances. See the migration guide for details. Particular notes:

Note: Apollo does not require using the add-webapollo-plugin.pl because the plugin is loaded implicitly by including the client/apollo/json/annot.json file at run time.

Upgrading existing JBrowse data stores

It is not necessary to change your existing JBrowse data directories to use Apollo 2.x, you can just point to existing data directories from your previous instances.

Adding custom CSS for track styling for JBrowse

There are a variety of different ways to include new CSS into the browser, but the easiest might be the following

Add the following statement to your trackList.json:

    "css" : "data/yourfile.css"

Then just place your CSS file in your organism's data directory.

Adding custom CSS globally for JBrowse

If you want to add CSS that is used globally for JBrowse, you can edit the CSS in the client/apollo/css folder, but since you need to re-deploy the app every time for updates, it is easier to just edit the data directories for your organisms (you do not need to re-deploy the app when you are editing organism specific data, since this is outside of the webapp directory and is not deployed with the WAR file)

Adding custom CSS globally for the GWT app

If you want to style the GWT sidebar, generally the bootstrap theme is used but extra CSS is also included from web-app/annotator/theme.css which overrides the bootstrap theme

Adding / using proxies

If you are https, or choose to use separate services rather than the default provided, you can setup a pass-through proxy or modify a particular URL.

This service is only available to logged-in users.

The internal proxy URL is:

<apollo url>/proxy/request/<encoded_proxy_url>/

For example if your URL the URL we want to proxy:

http://golr.geneontology.org/solr/select

encoded:

http%3A%2F%2Fgolr.geneontology.org%2Fsolr%2Fselect

If you user is logged-in and you pass in:

http://localhost/apollo/proxy/request/http%3A%2F%2Fgolr.geneontology.org%2Fsolr%2Fselect?testkey=asdf&anotherkey=zxcv

This will get proxied to:

http://golr.geneontology.org/solr/select?testkey=asdf&anotherkey=zxcv

If you choose to use another proxy service, you can go to the proxy service tab (as admin).

To use a proxy it has to be registered by an admin. Internally used proxies are provided by default.