Here we will introduce how to setup Apollo on your server. In general, there are two modes of deploying Apollo.
There is "development mode" where the application is launched in a temporary server (automatically) and there is
"production mode", which will typically require an external separate database and tomcat server where you can deploy the
This guide will cover the "development mode" scenario which is easy to start. See the setup guide to cover the "production mode" setup.
Installing grails is made easier by using SDKMAN (formerly GVM) which can automatically setup grails for you. We will use grails 2.4.5 for Apollo.
curl -s http://get.sdkman.io | bash
gvm install grails 2.4.5
It is not required but you can also install the groovy command line
gvm install groovy
Get the code
To setup Apollo, you can download the code from github:
- git clone https://github.com/GMOD/Apollo.git Apollo
- cd Apollo
Alternatively, download a .tar.gz file or .zip file from the releases page: https://github.com/GMOD/Apollo/releases.
There won't be any difference in the workflow either way that you choose to get the code.
Verify install requirements
We can now perform a quick-start of the application in "development mode" with this command:
The jbrowse and perl pre-requisites will be installed during this step, and if there is a success, then a temporary
server will be automatically launched at
Note: You can also supply a port number e.g.
apollo run-local 8085 if there are conflicts on port 8080.
Also note: if there are any errors at this step, check the setup.log file for errors. You can refer to the troubleshooting guide and often it just means the pre-requisites or perl modules failed.
Also also note: the "development mode" uses an in-memory H2 database for storing data by default. The setup guide will show you how to configure custom database settings.
Setting up the application
After we have a server setup, we will want to add a new organism to the panel. If you are a new user, you will want to setup this data with the jbrowse pre-processing scripts. You can see the data loading guide for more details, but essentially, you will want to load a reference genome and an annotations file at a minimum:
bin/prepare-refseqs.pl --fasta yourgenome.fasta --out /opt/apollo/data bin/flatfile-to-json.pl --gff yourannotations.gff --type mRNA \ --trackLabel AnnotationsGff --out /opt/apollo/data
Login to the web interface
After you access your application at http://localhost:8080/apollo/ then you will be prompted for login information
Figure 1. "Register First Admin User" screen allows you to create a new admin user.
Figure 2. Navigate to the "Organism tab" and select "Create new organism". Then enter the new information for your organism. Importantly, the data directory refers to a directory that has been prepared with the JBrowse data loading scripts from the command line. See the data loading section for details.
Figure 3. Open up the new organism from the drop down tab on the annotator panel.
If you completed this setup, you can then begin adding new users and performing annotations. Please continue to the setup guide for deploying the webapp to production or visit the troubleshooting guide if you encounter problems during setup.