Example Build Script on Unix with MySQL¶

This is an example build script. It may NOT be appropriate for your environment but does demonstrate what a typical build process might look like on a Unix system using MySQL.

Please consult our Setup and Configuration documentation for additional information.

# Install prereqs
apt-get install tomcat8 git ant openjdk-8-jdk nodejs
# Upped tomcat memory per Apollo devs instructions:
echo "export CATALINA_OPTS="-Xms512m -Xmx1g \
              -XX:+CMSClassUnloadingEnabled \
              -XX:+CMSPermGenSweepingEnabled \
              -XX:+UseConcMarkSweepGC" >> /etc/default/tomcat8

# Download and extract their tarball
npm install -g yarn
wget https://github.com/GMOD/Apollo/archive/2.5.0.tar.gz
mv 2.5.0.tar.gz Apollo-2.5.0.tar.gz
tar xf Apollo-2.5.0.tar.gz

Setup apollo mysql user and database¶

# Login to mysql e.g., 
mysql -u root
# Create a user 
CREATE USER 'apollo'@'localhost' IDENTIFIED BY 'THE_PASSWORD';
CREATE DATABASE `apollo-production`;
GRANT ALL PRIVILEGES ON `apollo-production`.* To 'apollo'@'localhost';

Configure apollo for mysql.¶

cd ~/src/Apollo-2.5.0
# Let's store the config file outside of the source tree.
mkdir ~/apollo.config
# Copy the template
cp sample-mysql-apollo-config.groovy ~/apollo.config/apollo-config.groovy 
ln -s ~/apollo.config/apollo-config.groovy
# For now, turn off tomcat8 so that we can see if the locally-run version works service tomcat8 stop
# Run the local version, which verifies install reqs, and does a bunch of stuff (see below) 
cd Apollo-2.5.0
./apollo run-local

If a pre-installed instance:¶

rm -rf /var/lib/tomcat/webapps/apollo
rm -f /var/lib/tomcat/webapps/apollo.war

Startup tomcat again:

service tomcat8 start

Create file target/apollo-2.5.0.war by running:

./apollo deploy    

and copy it into the war area where it is automatically:

sudo cp target/apollo-2.5.0.war /var/lib/tomcat/webapps/apollo.war

Prepare JBrowse data¶

Add the FASTA assembly:

~/src/Apollo-2.5.0/jbrowse/bin/prepare-refseqs.pl \
--fasta /research/dre/assembly/assembly1.fasta.gz \
--out ~/organisms/dre

Add annotations:

~/src/Apollo-2.5.0/jbrowse/bin/flatfile-to-json.pl \
--gff /research/dre/annotation/FINAL_annotations/ssc_v4.gff \ 
--type mRNA --trackLabel Annotations --out ~/organisms/dre

In interface point to directory ~/organisms/dre when loading organism.